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ptre tight vector  (Addgene inc)


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    Structured Review

    Addgene inc ptre tight vector
    Ptre Tight Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptre+tight+mir+1/pTRE-TIGHT+miR-1+(aka%3ApTRE-m1d)+(Plasmid+%2314896)/pm33067378-69-7-9
    Average 92 stars, based on 4 article reviews
    ptre tight vector - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Cloning:

    Article Title: Construction of a doxycycline inducible adipogenic lentiviral expression system
    Article Snippet: pLenti6/V5 lentiviral expression backbone was from Invitrogen. .. The templates for cloning TRE and TRE tight, pL3-TRE-LucGFP-2L (Plasmid 11685) and pTRE-Tight miR-1 (Plasmid 14896), respectively, and the template for cloning P aP2 and P′ aP2 , pBS-aP2 (Plasmid 11424) all came from Addgene. .. The template for cloning rtTA, pTet On was from Clontech and the template for cloning rtTA adv, PB-CA-rtTA advance was from Addgene.

    Plasmid Preparation:

    Article Title: Construction of a doxycycline inducible adipogenic lentiviral expression system
    Article Snippet: pLenti6/V5 lentiviral expression backbone was from Invitrogen. .. The templates for cloning TRE and TRE tight, pL3-TRE-LucGFP-2L (Plasmid 11685) and pTRE-Tight miR-1 (Plasmid 14896), respectively, and the template for cloning P aP2 and P′ aP2 , pBS-aP2 (Plasmid 11424) all came from Addgene. .. The template for cloning rtTA, pTet On was from Clontech and the template for cloning rtTA adv, PB-CA-rtTA advance was from Addgene.

    Article Title: Cell-specific CRISPR–Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins
    Article Snippet: .. The CMV-miR-1 expression vector was created by replacing the miR-122 coding sequence in vector CMV-miR-122 by the miR-1 coding sequence, which we obtained via restriction digest of pTRE_Tight_miR-1 (kind gift from David Bartel, Addgene plasmid #14896) with BamHI/HindIII. .. The Tet-inducible luciferase reporter and corresponding sgRNA construct (sgRNA1_Tet-inducible Luciferase reporter) were kind gifts from Moritoshi Sato (Addgene plasmids #64127 and #64161). dCas9-VP64_GFP was a kind gift from Feng Zhang (Addgene plasmid #61422).

    Article Title: Target molecules for transcriptional control systems
    Article Snippet: .. TREtight promoter was amplified from pTRE-TIGHT miR-1 (Addgene plasmid 14896), generating SphI and SbfI restriction sites on the 5′ end. ..

    Expressing:

    Article Title: Cell-specific CRISPR–Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins
    Article Snippet: .. The CMV-miR-1 expression vector was created by replacing the miR-122 coding sequence in vector CMV-miR-122 by the miR-1 coding sequence, which we obtained via restriction digest of pTRE_Tight_miR-1 (kind gift from David Bartel, Addgene plasmid #14896) with BamHI/HindIII. .. The Tet-inducible luciferase reporter and corresponding sgRNA construct (sgRNA1_Tet-inducible Luciferase reporter) were kind gifts from Moritoshi Sato (Addgene plasmids #64127 and #64161). dCas9-VP64_GFP was a kind gift from Feng Zhang (Addgene plasmid #61422).

    Sequencing:

    Article Title: Cell-specific CRISPR–Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins
    Article Snippet: .. The CMV-miR-1 expression vector was created by replacing the miR-122 coding sequence in vector CMV-miR-122 by the miR-1 coding sequence, which we obtained via restriction digest of pTRE_Tight_miR-1 (kind gift from David Bartel, Addgene plasmid #14896) with BamHI/HindIII. .. The Tet-inducible luciferase reporter and corresponding sgRNA construct (sgRNA1_Tet-inducible Luciferase reporter) were kind gifts from Moritoshi Sato (Addgene plasmids #64127 and #64161). dCas9-VP64_GFP was a kind gift from Feng Zhang (Addgene plasmid #61422).

    Amplification:

    Article Title: Target molecules for transcriptional control systems
    Article Snippet: .. TREtight promoter was amplified from pTRE-TIGHT miR-1 (Addgene plasmid 14896), generating SphI and SbfI restriction sites on the 5′ end. ..



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